Rvrsd-phs lqd chrmtgrphy (RP-LC) s md f lqd chrmtgrphy n whch nn-plr sttnry phs nd plr mbl phss r sd fr th sprtn f rgnc cmpnds. Th vst mjrty f sprtns nd nlyss sng hgh-prfrmnc lqd chrmtgrphy (HPLC) n rcnt yrs r dn sng th rvrsd phs md. n th rvrsd phs md, th smpl cmpnnts r rtnd n th systm th mr hydrphbc thy r.
Th fctrs ffctng th rtntn nd sprtn f slts n th rvrsd phs chrmtgrphc systm r s fllws:
. Th chmcl ntr f th sttnry phs, .., th lgnds bndd n ts srfc, s wll s thr bndng dnsty, nmly th xtnt f thr cvrg.
b. Th cmpstn f th mbl phs. Typ f th blk slvnts whs mxtrs ffct th plrty f th mbl phs, hnc th nm mdfr fr slvnt ddd t ffct th plrty f th mbl phs.
c. ddtvs, sch s bffrs, ffct th pH f th mbl phs, whch ffct th nztn stt f th slts nd thr plrty.
n rdr t rtn th rgnc cmpnnts n mxtrs, th sttnry phss, pckd wthn clmns, cnsst f hydrphbc sbstrts, bndd t th srfc f prs slc-gl prtcls n vrs gmtrs (sphrc, rrglr), t dffrnt dmtrs (sb-2, 3, 5, 7, 10 m), wth vryng pr dmtrs (60, 100, 150, 300, ). Th prtcl's srfc s cvrd by chmclly bndd hydrcrbns, sch s C3, C4, C8, C18 nd mr. Th lngr th hydrcrbn ssctd wth th sttnry phs, th lngr th smpl cmpnnts wll b rtnd. Sm sttnry phss r ls md f hydrphbc plymrc prtcls, r hybrdzd slc-rgnc grps prtcls, fr mthd n whch mbl phss t xtrm pH r sd. Mst crrnt mthds f sprtn f bmdcl mtrls s C-18 clmns, smtms clld by trd nms, sch s DS (ctdcylsln) r RP-18.
Th mbl phss r mxtrs f wtr nd plr rgnc slvnts, th vst mjrty f whch r mthnl nd ctntrl. Ths mxtrs slly cntn vrs ddtvs sch s bffrs (ctt, phspht, ctrt), srfctnts (lkyl mns r lkyl slfnts) nd spcl ddtvs (DT). Th gl f sng spplmnts f n knd r nthr s t ncrs ffcncy, slctvty, nd cntrl slt rtntn.